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RP - Generation and purification of rabbit or goat polyclonal antibodies (»Add to Infobox)

Melchior Frauke

Frauke Melchior's Group

Frauke Melchior
ZMBH, Heidelberg, Germany
TABLE OF CONTENT
1. Generation of antigen
2. Generation of emulsion for injection
3. Injection / bleeding scheme for goats or rabbits
4. From blood to serum
5. Test of serum / purified antibodies by western blotting
6. Affinity purification
7. Supplementary protocol: reduction of peptide



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Melchior Frauke
(Principal Investigator / Frauke Melchior's Group)



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Generation and purification of rabbit or goat polyclonal antibodies


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