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RP - Purification of untagged recombinant human RanGAP1
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RanGAP1 was the first SUMO target identified. It is very efficiently sumoylated just in the presence of E1 and E2 enzymes, without the need for an E3 ligase. The sumoylation of RanGAP1 is essential to target it to the cytoplasmic periphery of the nuclear pore complex via interaction with RanBP2 (Nup358).
Bacterially expressed RanGAP1 is purified from inclusion bodies and refolded to its catalytically fully active form during a dialysis step. Properly folded (active) RanGAP1 can be separated from the unfolded (inactive) form by gel filtration.
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Purification of untagged recombinant human RanGAP1
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