General description:
Our lab has contributed to the characterization of two protein:protein interaction networks, the EH and the mono-ubiquitin (Ub) networks, which play a major role in the endocytic route and also intersect many other cellular functions, including RTK signaling, regulation of cytoarchitecture, control of DNA repair and of transcription. We aim to compile physical and functional maps of the EH and monoUb networks, and to translate these maps (reverse-engineering) into actual functional programs. We integrate predictions stemming from functional genomics approaches with more conventional ad hoc lines of experimentation (genetics, cell biology, cellular biochemistry) in order to unravel the relationships between endocytosis, signaling, and cancer. Presently three major levels of efforts are being pursued in the lab:
1.
| The EH network in physiology and in cancer
| 2.
| Ub-based networks and cancer
| 3.
| Endocytosis as a process and cancer |
Role in RUBICON:
 | Identification and characterization of the monoubiquitin proteome.
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| Identification of the molecular circuitries through which receptor tyrosine kinases lead to monoubiquitination of intracellular proteins.
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| Analysis of the physiological role of monoubiquitination in model proteins.
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| Expression analysis of ubiquitinated proteins in physiological and pathological conditions. |
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