In this review, Rosine Haguenauer-Tsapis and collaborators discuss recent examples illustrating the role of Rsp5p in membrane protein trafficking and providing new insight into the regulation of this ubiquitin ligase E3 by adaptor proteins. Open the download file and learn in detail the role of Rsp5p in membrane protein trafficking: i) endocytosis; ii) sorting to multivesicular bodies and iii) from the Golgi to the endosome compartment.