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General description:
The Crystallisation Facility at The Netherlands Cancer Institute (NKI) is part of the Structural Biology groups headed by Anastassis Perrakis and Titia Sixma. The facility provides a service for crystallisation of biomolecular samples including proteins, DNA, RNA and macromolecular complexes. Crystallisation service is offered only to people working at projects within the NKI or to collaborating research programs, including the European projects RUBICON, 3D-Repertoire, and BioXHit.
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Specific service:
Within RUBICON, Partner NKI offers initial crystallisation screening for purified protein (provided by partner labs) in two different formats.
 | High-throughput “classical” crystallisation screens with max. 8 plates (96 conditions each) using standardized conditions. The “Crystal farm”, an automated storage and imaging system has capacities for 400 plates at a time and includes a web-based interface enabling access to imaging of individual crystallisation experiments thus allowing monitoring the progress of crystallisation online.
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| Automated microfluidics crystallisation system “TOPAZ” based on free interface diffusion (FID) to identify optimal crystallisation conditions with 96 individual conditions /screen. Due to high cost this screening method is recommended only if either the classical approach failed, or when the protein is available only in minute (< 10 µl) amounts.
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To access the Core facility files (Rubicon-members only!):
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| scroll to the bottom of this page and click 'login'
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| Click on the 'documents' drop down menu (top right hand side), and select the 'core facility' folder. Within this folder you will find the Crystallisation folder.
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Core Facilities
Crystallisation