RP - Protocol for siRNA transfection into primary human cells (»Add to Infobox)

Dejean Anne

Anne Dejean's Group

Efficiency of transfection into primary cells is a well known problem limiting experimental design, and available methods such as amaxa biosystems depend on usage of large quantities of cells. In addition, the procedure itself causes stress for cells making it hard to study certain phenotypes like cellular senescence. We used WI38 normal human fibroblasts as our experimental system and performed experiments using scrambled siRNA smart pool in order to set up our controls for possible defects of manipulations. We optimized the conditions for using Dharmacon siRNA SMART pools in 6-well plates. Using this transfection reagent and fluroescencent siRNA control, we visualized the delivery of siRNAs into primary cells at a 99% of efficiency.

MEMBERS (1/1)

Dejean Anne (Principal Investigator / Anne Dejean's Group)

DOWNLOADS (1/1)