RP - How to determine the type of ubiquitin formed chains on your favorite substrate (»Add to Infobox)

Israel Alain, Professor

Alain Israël's Group

In order to determine the type of ubiquitin chain formed on Notch or on Deltex, we generated expression vectors encoding for tagged ubiquitin mutants. These vectors have to be transfected together with the expression vector encoding the protein of interest, then the ubiquitinated proteins are immunoprecipitated and the ubiquitinated forms are detected by anti-VSV western blotting. Note that the extracts and the IPs are done in non-denaturing conditions, therefore several controls are necessary to be sure that the ubiquitinated products derive from the immunoprecipitated proteins, and not from co-immunoprecipitated factors.

MEMBERS (1/1)

Israel Alain, Professor (Principal Investigator / Alain Israël's Group)

DOWNLOADS (1/1)