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RP - Purification of the yeast HRD-ligase complex by immunoprecipitation from solubilized microsomes
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Thomas Sommer
Max-Delbrük-Center for Molecular Medicine, Berlin, Germany
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This protocol is used by the Sommer-lab to purify the HRD-ligase complex from yeast microsomes for subsequent analysis by immunoblotting. It may be up-scaled and the samples may then be analyzed by mass-spectroscopy. You need a yeast strain expressing myc- or HA-epitope tagged variants of subunits of the HRD-ligase.
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Purification of the yeast HRD-ligase complex by immunoprecipitation from solubilized microsomes
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