RP - Anti-Ubiquitin western blot (»Add to Infobox)

Di Fiore Pier Paolo, Polo Simona

Pier Paolo Di Fiore's Group, Simona Polo's group

This protocol was set-up to increase the sensitivity of anti-Ub western-blot. It consists in a denaturing step of the proteins directly on the filter, which renders the epitopes more accessible to the antibodies increasing the ratio signal to noise.

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Di Fiore Pier Paolo (Principal Investigator / Pier Paolo Di Fiore's Group)

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