RP - Epidermal growth factor receptor (EGFR) downmodulation assay (»Add to Infobox)

Di Fiore Pier Paolo, Polo Simona

Pier Paolo Di Fiore's Group, Simona Polo's group

With this assay the disappearance of the receptor from the cell surface is followed upon long exposure with saturant EGF concentration. Alterations in the normal balance between degradation and recycling of the receptor can be unmasked. Data should be plotted as percentage of surface EGFR/initial amount at zero time point (Y-axes) versus time (X-axes). In HeLa cells, after 60 min almost all the EGFR is downmodulated, with less than 10% remaining at the cell-surface.

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Di Fiore Pier Paolo (Principal Investigator / Pier Paolo Di Fiore's Group)

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