RP - Epidermal growth factor receptor (EGFR) recycling assay (»Add to Infobox)

Di Fiore Pier Paolo, Polo Simona

Pier Paolo Di Fiore's Group, Simona Polo's group

This assay allows to have a quantitative assessment of the recycling rate of EGFR, by measuring the amount of intact ligand that is retrieved in the medium upon time. Data should be plotted as fraction of intact EGF in the medium/initial amount at zero time point (Y-axes), versus time (X-axes). In HeLa cells, after 1 h the curve reaches a plateau (around 40% recycling), meaning that all ligand that was initially internalized, has been extinguished (either recycled or degraded).

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Di Fiore Pier Paolo (Principal Investigator / Pier Paolo Di Fiore's Group)

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