RP - Phosphatases and deglycosidases assays for EGFR (»Add to Infobox)

Di Fiore Pier Paolo, Polo Simona

Pier Paolo Di Fiore's Group, Simona Polo's group

EGFR, as many plasma membrane receptors, is heavily glycosilated in the biosynthetic pathway. It also becomes phosphorylated at different Tyrosine and Serine/Threonine residues upon EGF stimulation. This leads to a consistent shift in its molecular weight and to the generation of a smeary pattern in SDS-PAGE gels. This protocol allows the almost complete dephosphorylation and deglycosidation of the receptor in order to reduce the smear at one single sharp band. This allows, for example, a better analysis of the ubiquitination pattern of the EGFR or other proteins of interest.

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Di Fiore Pier Paolo (Principal Investigator / Pier Paolo Di Fiore's Group)

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