RP - In vitro deUbiquitination assay with purified GST-DUBs (»Add to Infobox)

Stefano Gastaldello and Maria G. Masucci
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.


This protocol is designated to detect the activity of a purified GST-deubiquitinating enzyme with a pool of poli-ubiquitinated conjugated proteins in sepharose beads.

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Masucci Maria Grazia (Principal Investigator / Maria Masucci's Group)

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In vitro deUbiquitination assay with purified GST-DUBs

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