RP - Luciferase-reporter based DUB screen (»Add to Infobox)

Bernards Rene, Professor

Rene Bernards’ Group

Components of a signalling pathway may be either activated or inactivated by covalent attachment of one or more ubiquitin moieties. The activity of such signal transduction pathways can often be measured using reporter genes e.g. the Firefly/Renilla dual-luciferase reporter system. In order to specifically study the involvement of de-ubiquitinating enzymes (DUBs) in signalling pathways, we have constructed a short hairpin RNA (shRNA) library targeting the gene family of human DUBs. Co-transfection of the DUB library can be used in combination with various reporters to identify DUBs that affect the activity of any chosen pathway under various conditions.

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Bernards Rene, Professor (Principal Investigator / Rene Bernards’ Group)

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