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RP - Generation and purification of rabbit or goat polyclonal antibodies
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Frauke Melchior
ZMBH, Heidelberg, Germany
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TABLE OF CONTENT
1. Generation of antigen
2. Generation of emulsion for injection
3. Injection / bleeding scheme for goats or rabbits
4. From blood to serum
5. Test of serum / purified antibodies by western blotting
6. Affinity purification
7. Supplementary protocol: reduction of peptide
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Generation and purification of rabbit or goat polyclonal antibodies
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