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RP - Filter aided proteome preparation (FASP)
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Matthias Mann
Max Planck Institute for Biochemistry, Department of Proteomics and Signaltransduction, Martinsried, Germany
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In-gel digestion for mass spectrometry-based proteomics is extremely robust whereas in-solution digestion is more easily automated and minimizes sample handling. Filter aided proteome preparation (FASP), combines theses advantages by completely solubilizing the proteome in sodium dodecyl sulfate (SDS), which is exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter are pure, allowing single run analysis of organelles and unprecedented depth of proteome coverage.
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Filter aided proteome preparation (FASP)
Matthias
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