RP - Immunoprecipitation of yeast proteins in denaturing conditions for analysis of ubiquitylation - METHOD 2 (»Add to Infobox)

Sébastien Léon and Rosine Haguenauer-Tsapis
Centre Nationale de la Recherche Scientifique, Paris, France


The aim of this experiment is to immunoprecipitate proteins from yeast in denaturing conditions. After blotting of the immunoprecipitate with anti-Ubiquitin antibodies (ex. clone P4D1), this allows to verify the ubiquitylation status of a given protein. This protocol is adapted from Kiel et al. 2005.

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Haguenauer-Tsapis Rosine (Principal Investigator / Rosine Haguenauer-Tsapis's Group)

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