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RUBICON Protocols Database
NEWS  (35)

The following protocols have been tested and reviewed by the RUBICON Network of Excellence. It is our hope that they will smooth the research in the field of the ubiquitin and ubiquitin-like modifiers



35 News related to RUBICON Protocols Database
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RP - 125I-EGF and 125I-Tf internalization assays
(Di Fiore Pier Paolo)

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RP - Anti-Ubiquitin western blot
(Di Fiore Pier Paolo)

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RP - Cycloheximide-decay assay
(Sommer Thomas)

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RP - Epidermal growth factor receptor (EGFR) downmodulation assay
(Di Fiore Pier Paolo)

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RP - Epidermal growth factor receptor (EGFR) recycling assay
(Di Fiore Pier Paolo)

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RP - Expression and purification of SUMO E1 (His-Aos1/Uba2 or His-Aos1/Uba2-His)
(Melchior Frauke)

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RP - Expression and purification of Ubc9 (SUMO E2)
(Melchior Frauke)

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RP - Filter aided proteome preparation (FASP)
(Mann Matthias)

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RP - Generation and purification of rabbit or goat polyclonal antibodies
(Melchior Frauke)

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RP - Generation of 32P-labeled ubiquitin, SUMO & Nedd8
(Scheffner Martin)

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RP - Growth Hormone Receptor Pull-down with biotinylated Growth Hormone
(Strous Ger)

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RP - How to determine the type of ubiquitin formed chains on your favorite substrate
(Israel Alain, Professor)

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RP - Immunofluorescence assays for EGFR and TfR
(Di Fiore Pier Paolo)

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RP - Immunoprecipitation of yeast proteins in denaturing conditions for analysis of ubiquitylation - METHOD 1
(Haguenauer-Tsapis Rosine)

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RP - Immunoprecipitation of yeast proteins in denaturing conditions for analysis of ubiquitylation - METHOD 2
(Haguenauer-Tsapis Rosine)

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RP - In Gel Digestion for Mass Spectrometry (MS) Analysis
(Mann Matthias)

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RP - In Solution Digest for Mass Spectrometry (MS) Analysis
(Mann Matthias)

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RP - In vitro deUbiquitination assay with purified GST-DUBs
(Masucci Maria Grazia)

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RP - In vitro polyubiquitination assay
(Wolf Dieter H.)

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RP - In vitro ubiquitination assay
(Di Fiore Pier Paolo)

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RP - Large-scale in vitro PCNA ubiquitinylation by Rad18/Rad6
(Sixma Titia)

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RP - Luciferase-reporter based DUB screen
(Bernards Rene, Professor)

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RP - N-terminal ubiquitination and general techniques for monitoring ubiquitin conjugation and protein degradation
(Ciechanover Aaron, Professor)

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RP - Phosphatases and deglycosidases assays for EGFR
(Di Fiore Pier Paolo)

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RP - pIKBa ubiquitination assay using purified SCF complex
(Ben-Neriah Yinon)

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